Step-up method for COLD-PCR enrichment
作者： Reyes Candau-Chacon
DOI:
关键词: High homology 、 COLD-PCR 、 Genetics 、 Fold (higher-order function) 、 Biology 、 Polymerase 、 Enrichment methods 、 Computational biology
摘要: Methods of using polymerase chain reactions to enrich a target sequence in sample containing reference sequences and having high homology amplifiable by the same primer pair are provided herein. In particular methods provide robust means improve fold enrichment minimize reaction-to-reaction, well-to-well run-to-run variations methods.
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