T790M mutation detection method based on nested composite COLD-PCR
作者： Liu Hao , Luo Kai , Qiu Qinwei , Qiu Ni , Zhang Zhijie
DOI:
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摘要: The invention discloses a T790M mutation detection method based on nested composite COLD-PCR combination sequencing. uses sample nucleic acid to be measured as template, and performs sequencing by means of primers shown in SEQ ID No.1-4 No.5-8. comprises an outer reaction inner reaction, wherein the is combined routine PCR reaction. has good sensitivity, lower limit repeatability, can amplify wild type mutant samples undifferentiated manner, enrichment effect, adjust effect sensitivity according need adjusting cycle number ratio very application prospect.
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