Magic-angle spinning NMR of cold samples.

摘要: Magic-angle-spinning solid-state NMR provides site-resolved structural and chemical information about molecules that complements many other physical techniques. Recent technical advances have made it possible to perform magic-angle-spinning experiments at low temperatures, allowing researchers trap reaction intermediates studies of low-temperature phenomena such as quantum rotations, tunneling, ortho-para conversion between spin isomers, superconductivity. In examining biological molecules, the improved sensitivity provided by cryogenic facilitates study protein assembly or membrane proteins. The combination low-temperatures with dynamic nuclear polarization has potential boost even further. Many research groups, including ours, addressed challenges developed hardware for samples cooled down a few tens degrees Kelvin. this Account, we briefly describe these developments review several recent activities our group which involve NMR. Low-temperature operation allows us cannot be studied under ambient conditions because their short lifetime. We used electronic structure bathorhodopsin, primary photoproduct light-sensitive protein, rhodopsin. This project custom-built probe in presence illumination (the image shows illuminated spinner module). also technique behavior within restricted environment. Small-molecule endofullerenes are interesting molecular systems rotors confined well-insulated, well-defined, highly symmetric discuss how solid state can give on dynamics ortho-water fullerene cage. Molecular motions often connected fundamental properties; therefore, an understanding important fields ranging from material science biochemistry. present case ibuprofen sodium salt exhibits different conformational freedom parts same molecule, leading range line broadening narrowing function temperature.