Assessment of the classical MDR phenotype in epithelial ovarian carcinoma using primary cultures: a feasibility study.

摘要: This in vitro feasibility study has assessed a number of techniques and their applicability when looking at the role multidrug resistance (MDR) solid tumours. Fresh tumour material was obtained from 34 patients, (11 previously treated, 23 untreated) with ovarian adenocarcinoma. Doxorubicin sensitivity measured using MTT assay +/- cyclosporins, Pgp expression by immunocytochemistry MRK-16 MoAb flow cytometry used to assess intracellular drug accumulation PSC 833. 85% samples showed some evidence modest chemosensitisation cyclosporins (median 1.74-fold). We saw marked variation positive cells between patients (1-87%, median 31%). 63% tested an enhancement DNR presence 833, increase 7% (sample range 0-29%). The present highlights technical difficulties encountered working fresh ex vivo. conclude that screening for suitability enter clinical trials incorporating MDR modulating agents is technically demanding, but feasible.