Quantitative and functional assay of MDR1/P170-mediated MDR in ascites cells of patients with ovarian cancer.
作者： Zoárd Tibor Krasznai , Elza Friedländer , András Nagy , Gábor Szabó , György Vereb
DOI:
关键词:
摘要: Background: MDR1-associated P-glycoprotein- dependent multidrug resistance is a common cause of chemotherapy failure in patients with advanced ovarian cancer. Here, we describe clinical method for simultaneously assessing the expression and function MDR1/Pgp tumour cells from ascites malignant carcinoma. Materials Methods: Cells 35 were collected. The Pgp detected by flow cytometry. For functional study, rhodamine 123 was used. Results: Using Pgp- specific UIC2 MM6.15 antibodies, demonstrated presence 10-79 % (38.9±20, 7; n=35) CA125-positive cell subpopulations. results assay showed strong correlation level (r=0.976; p=3.2x10 -5 ). Conclusion: Direct detection provide useful information more efficient treatment diseases proper adjustment chemotherapeutic protocol. second most frequent gynaecological malignancy carcinoma (1). Ovarian cancer spreads early disease to peritoneal cavity. often remain confined peritoneum an en bloc resection usually not possible (2). Therefore, surgery combined accepted approach treating at later stages. major problem
参考文章(21)
Mark M. Baekelandt, Gunnar B. Kristensen, Ruth Holm, Jahn M. Nesland, Claes G. Tropé, P-glycoprotein expression is a marker for chemotherapy resistance and prognosis in advanced ovarian cancer. Anticancer Research. ,vol. 20, pp. 1061- 1067 ,(2000)
Jean M. Sargent, Helen M. Coley, Christine J. Williamson, Colin G. Taylor, Sarah E. Gregson, Jenny Titley, Grazyna M. Lewandowicz, Alena W. Elgie, Rik J. Scheper, Assessment of the classical MDR phenotype in epithelial ovarian carcinoma using primary cultures: a feasibility study. Anticancer Research. ,vol. 22, pp. 69- 74 ,(2002)
Anne W. Hamburger, José V. Ordóñez, Allison M. Sisk, Richard K. Wu, Richard E. Nora, Christopher C. Joneckis, Douglas D. Ross, Estimation of cell survival by flow cytometric quantification of fluorescein diacetate/propidium iodide viable cell number. Cancer Research. ,vol. 49, pp. 3776- 3782 ,(1989)
Christine A. Hrycyna, Wilma Y. Medina-Pérez, Robert W. Robey, Michael Dean, Anne van de Laar, Yasumasa Honjo, Thomas Litman, Qing-Wu Yan, Susan E. Bates, Acquired mutations in the MXR/BCRP/ABCP gene alter substrate specificity in MXR/BCRP/ABCP-overexpressing cells Cancer Research. ,vol. 61, pp. 6635- 6639 ,(2001)
D M Bradshaw, R J Arceci, Clinical relevance of transmembrane drug efflux as a mechanism of multidrug resistance. Journal of Clinical Oncology. ,vol. 16, pp. 3674- 3690 ,(1998) , 10.1200/JCO.1998.16.11.3674
L Homolya, M Holló, M Müller, EB Mechetner, B Sarkadi, A new method for quantitative assessment of P-glycoprotein-related multidrug resistance in tumour cells British Journal of Cancer. ,vol. 73, pp. 849- 855 ,(1996) , 10.1038/BJC.1996.151
Naeem Aslam, Christopher R. Marino, Malignant Ascites Archives of Internal Medicine. ,vol. 161, pp. 2733- 2737 ,(2001) , 10.1001/ARCHINTE.161.22.2733
Éva Karászi, Katalin Jakab, László Homolya, Gergely Szakács, Zsolt Holló, Béla Telek, Attila Kiss, László Rejtô, Sarolta Nahajevszky, Balázs Sarkadi, János Kappelmayer, Calcein assay for multidrug resistance reliably predicts therapy response and survival rate in acute myeloid leukaemia. British Journal of Haematology. ,vol. 112, pp. 308- 314 ,(2001) , 10.1046/J.1365-2141.2001.02554.X
Hiroaki Kajiyama, Fumitaka Kikkawa, Osamu Maeda, Takahiro Suzuki, Kazuhiko Ino, Shigehiko Mizutani, Increased expression of dipeptidyl peptidase IV in human mesothelial cells by malignant ascites from ovarian carcinoma patients. Oncology. ,vol. 63, pp. 158- 165 ,(2002) , 10.1159/000063801
Ter�z M�ri�n, L�szl� Balkay, Zolt�n Krasznai, Lajos Tr�n, Membrane permeability changes induce hyperpolarization in transformed lymphoid cells under high-density culture conditions. Cytometry. ,vol. 41, pp. 186- 192 ,(2000) , 10.1002/1097-0320(20001101)41:3<186::AID-CYTO5>3.0.CO;2-J