MDR1 and MDR3 genes and drug resistance to cisplatin of ovarian cancer cells
作者： Lirong Ren , Lan Xiao , Jianli Hu , Zhimin Li , Zehua Wang
DOI: 10.1007/S11596-007-0627-7
关键词: P-glycoprotein 、 Small interfering RNA 、 Viability assay 、 Transfection 、 Cisplatin 、 Molecular biology 、 Flow cytometry 、 Cell culture 、 Cancer research 、 Biology 、 Apoptosis 、 Genetics 、 Biochemistry 、 Biomaterials 、 Biomedical engineering
摘要: To investigate the relationship between MDR1 and MDR3 gene drug resistance to cisplatin of ovarian cancer cells. Two siRNAs (MDR1, MDR3) which specifically targeted genes were transfered into A2780/DDP Then double staining with Annexin-V-FITC/PI was used detect cell apoptosis by flow cytometry (FCM). viability determined MTT. mRNA assessed RT-PCR. Caspase-3 protein detected Western blotting. Transfection siRNA cells failed reverse drug-resistance (P>0.05). No significant difference in efficiency observed siRNA, pSuppressorNeo vector transfection untreated In presence different concentrations, not significantly decreased after transfection. changes found siRNA-transfected As compared groups, no existed expression The caspase-3 transfected increased. It is concluded that multidrug induced carcinoma lines due overexpression gene. mediated P-glycoprotein.
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