Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis.

摘要: The purpose of this study was to determine whether or not membrane-bound and soluble forms IL-4 receptors are expressed by isolated subsets murine lung fibroblasts evaluate the potential functional consequences receptor triggering. Recent studies demonstrate that IL-4-synthesizing Th2 cells mast present in increased numbers during inflammation fibrosis, suggesting may play a regulatory role these events. We hypothesize pulmonary thereof intimately involved inflammatory response is an active player stimulating fibroblast collagen synthesis hyperproliferation, creating fibrotic environment lung. used experiments differ only surface expression thymocyte-1 (Thy-1) Ag, but also function morphology. now report novel finding at discordant levels on Thy-1+ Thy-1- fibroblasts. IL-4R level affinity were analyzed using monoclonal anti-IL-4R Ab equilibrium binding analysis with 125I-labeled IL-4. Reverse transcriptase PCR demonstrated presence mRNA for IL-4R. Lung secrete protein dramatically different levels, as detected ELISA. treated cytokine profibrotic. responded proliferating up-regulating production. In contrast, proliferate lesser degree than stimulated collagen. Overall, results suggest elevated site injury could result development fibrosis enhancing subset proliferation synthesis.