Events Involving the Nucleoskeleton and Cytoskeleton

摘要: In chapters 1 and 2 we argued that as a matter of principle processes within the nuclear cytoplasmic compartments must be significant components transport. chapter 4 found assuming opposite position, i.e., presuming events at envelope are only ones relevant to nucleocytoplasmic transport, led ambiguities real interpretation difficulties. Evidently consider roles structures major cell if approach an adequate overall understanding, here discussion ‘characterization’ in 3 will become relevant. However, re-emphasize evidence for (say) involvement cytoskeletal element given transport process could interpreted many ways. Even experimental artifacts excluded, fibrillar might: (a) act ‘railroad’ transport; (b) serve assembly site transportant-containing complexes or processing final anchoring them; (c) sequester transportants corequisites by specific binding nonspecific association; (d) exclude corequisites, confining them aqueous channels; (e) generate fluid flow such channels. These alternatives not mutually exclusive there may others. Also, lack free mobility cytoplasm does necessarily imply (direct) association with membrane (chapter 2). short, this aspect is interpretative minefield it advisable proceed caution. With regard compartment situation even more fraught because nucleoskeleton so much less well characterized than cytoskeleton.