A three-dimensional view of precursor messenger RNA metabolism within the mammalian nucleus
作者： K. Carter , D Bowman , W Carrington , K Fogarty , J. McNeil
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摘要: A quantitative three-dimensional analysis of nuclear components involved in precursor messenger RNA metabolism was performed with a combination fluorescence hybridization, immunofluorescence, and digital imaging microscopy. Polyadenylate [poly(A)] RNA-rich transcript domains were discrete, internal regions that formed ventrally positioned horizontal array monolayer cells. dimmer, sometimes strand-like, poly(A) signal dispersed throughout the nucleoplasm. Spliceosome assembly factor SC-35 localized within center individual domains. These data support model which there is specific topological arrangement noncontiguous centers metabolism, from transport toward envelope radiates.
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D.J. Elliott, D.S. Bowman, N. Abovich, F.S. Fay, M. Rosbash, A yeast splicing factor is localized in discrete subnuclear domains. The EMBO Journal. ,vol. 11, pp. 3731- 3736 ,(1992) , 10.1002/J.1460-2075.1992.TB05458.X
M. Carmo-Fonseca, D. Tollervey, R. Pepperkok, S. M. Barabino, A. Merdes, C. Brunner, P. D. Zamore, M. R. Green, E. Hurt, A. I. Lamond, Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery. The EMBO Journal. ,vol. 10, pp. 195- 206 ,(1991) , 10.1002/J.1460-2075.1991.TB07936.X
Carol Villnave Johnson, Robert H. Singer, Jeanne Bentley Lawrence, Chapter 3 Fluorescent Detection of Nuclear RNA and DNA: Implications for Genome Organization Methods in Cell Biology. ,vol. 35, pp. 73- 99 ,(1991) , 10.1016/S0091-679X(08)60569-5
I Pettersson, M Hinterberger, T Mimori, E Gottlieb, J A Steitz, The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies. Journal of Biological Chemistry. ,vol. 259, pp. 5907- 5914 ,(1984) , 10.1016/S0021-9258(18)91101-4
S. Fakan, E. Puvion, The Ultrastructural Visualization of Nucleolar and Extranucleolar RNA Synthesis and Distribution International Review of Cytology-a Survey of Cell Biology. ,vol. 65, pp. 255- 299 ,(1980) , 10.1016/S0074-7696(08)61962-2
David L Spector, XIANG-DONG Fu, T Maniatis, Associations between distinct pre-mRNA splicing components and the cell nucleus. The EMBO Journal. ,vol. 10, pp. 3467- 3481 ,(1991) , 10.1002/J.1460-2075.1991.TB04911.X
Walter A. Carrington, Image restoration in 3-D microscopy with limited data Bioimaging and Two-Dimensional Spectroscopy. ,vol. 1205, pp. 72- 83 ,(1990) , 10.1117/12.17785
J.D. Northway, E.M. Tan, Differentiation of antinuclear antibodies giving speckled staining patterns in immunofluorescence Clinical Immunology and Immunopathology. ,vol. 1, pp. 140- 154 ,(1972) , 10.1016/0090-1229(72)90013-X
D. L. Spector, Higher order nuclear organization: three-dimensional distribution of small nuclear ribonucleoprotein particles Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 147- 151 ,(1990) , 10.1073/PNAS.87.1.147
A. Krystosek, T. T. Puck, The spatial distribution of exposed nuclear DNA in normal, cancer, and reverse-transformed cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 6560- 6564 ,(1990) , 10.1073/PNAS.87.17.6560