Differential T Cell-Mediated Regulation of CD23 (FcεRII) in B Cells and Follicular Dendritic Cells

摘要: Differences in murine follicular dendritic cells (FDC)-CD23 expression under Th1 vs Th2 conditions prompted the hypothesis that T help regulate phenotype of FDCs. FDCs express CD40, suggesting cell-CD40L and lymphokines may be involved regulating FDC-CD23. To test this, highly enriched were incubated with CD40L trimer or anti-CD40 to mimic cell signaling presence IFN-γ IL-4. Surface CD23 was determined by flow cytometry, whereas mRNA levels its isoforms CD23a CD23b independently measured quantitative PCR. When either agonistic Ab, FDC-CD23 increased both at protein levels. Moreover, engagement FDC-CD40 enhanced for isoforms. In addition, substantially CD40-stimulated Curiously, IL-4 could also up-regulate FDC-CD23a but not -CD23b. Anti-IFN-γ dramatically inhibited mice immunized CFA, anti-IL-4 had only a modest inhibitory effect. contrast FDCs, surface B cell-CD23 as well -CD23b, message expression. short, regulated very differently cells. Previous studies suggest high inhibit IgE production, this CD40L-mediated up-regulation explain, least part, why responses are associated low vivo.