Expression and Functional Analysis of the BCL2-Associated Agonist of Cell Death (BAD) Gene in the Sheep Ovary During the Reproductive Cycle

摘要: Most ewes in China are seasonally polyestrous with normal ovulatory cycles, which is controlled by photoperiod (length of the daily light phase). These estrous short-day season and anestrus long-day cannot mate during anestrus. Thus seasonal breeding limits both diversification intensification production. If sheep can estrus all round year, it be mated twice per greatly improve economic benefits. To change at genetic level cultivating new breeds, important to understand molecular mechanisms trait sheep. The BCL2-associated agonist cell death (BAD) gene being a regulator cellular apoptosis was identified our previous RNA-Seq, associated follicular development mammalian ovaries. However, mechanism how BAD regulate poorly understood. In this study, we characterized ovine BAD, including full-length mRNA cloning protein sequence prediction, as well expression profile Small-tailed Han (STH) highest levels were observed hypothalamus, lung, pituitary, while lowest liver. Functional analysis performed primary granulosa cells concentration P4 significantly increased after RNAi interference shown opposite overexpression vitro. It has been found that reduce progesterone promoting ovarian GC apoptosis, might involved regulating cycle