Localization of NADPH oxidase subunits in neonatal sympathetic neurons
作者： Eric W. Hilburger , Evan J. Conte , Dennis W. McGee , Steven P. Tammariello
DOI: 10.1016/J.NEULET.2004.11.066
关键词: Protein subunit 、 Superior cervical ganglion 、 Oxidase test 、 Biochemistry 、 Neuron 、 Cytoplasm 、 Intracellular 、 NADPH oxidase 、 Nerve growth factor 、 Cell biology 、 Biology 、 General Neuroscience
摘要: Abstract Reactive oxygen species (ROS) trigger programmed cell death in neonatal sympathetic neurons that have been deprived of nerve growth factor (NGF), however, the source these intermediates has not established. Using laser scanning confocal microscopy (LSCM), intracellular distribution subunits ROS-generating enzyme NADPH oxidase was examined superior cervical ganglion (SCG). Optical sectioning using LSCM showed gp91-phox and p22-phox co-localize at membrane, while p47-phox p67-phox are found uniformly distributed cytoplasm maintained presence NGF. Within 4 h after NGF deprivation, both exhibit punctate staining membrane. Furthermore, a sub-population cytosolic appeared to with membrane-bound NGF-deprived neurons. These data provide support for suggest this may become activated following withdrawal
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