Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor.

摘要: The time course of molecular events that accompany degeneration and death after nerve growth factor (NGF) deprivation neuroprotection by NGF other agents was examined in cultures NGF-dependent neonatal rat sympathetic neurons compared to apoptosis. Within 12 h onset deprivation, glucose uptake, protein synthesis, RNA synthesis fell precipitously followed a moderate decrease mitochondrial function. mechanisms underlying the deprivation-induced neuronal were found be different, demonstrating this is insufficient cause subsequently. After these early changes during atrophy, inhibition ceased halt while readdition or cAMP analogue remained neuroprotective for 6 h. This suggests model which putative killer reaches lethal levels several hours before cease respond with survival become committed die. Preceding loss viability 5 concurrent commitment die, DNA fragmented into oligonucleosomes. temporal pharmacological characteristics fragmentation consistent being part mechanism commits neuron antimitotic neurotoxin cytosine arabinoside induced presence NGF, supporting previous evidence it mimicked closely. Thus trophic occurs apoptosis an example programmed cell death.