Oncogene alterations in carcinomas of the uterine cervix: overexpression of the epidermal growth factor receptor is associated with poor prognosis.
作者： A.-M. F. Kersemaekers , M. J. Van De Vijver , L. J. C. M. Van Den Broek , J. Hermans , G. J. Fleuren
DOI:
关键词: Lymph node 、 Epidermal growth factor receptor 、 Carcinoma 、 Biology 、 Cancer 、 Epidermal growth factor 、 Oncogene 、 Immunohistochemistry 、 Cervical cancer 、 Cancer research
摘要: The involvement of human papillomavirus (HPV) in the development carcinomas uterine cervix has been firmly established. However, other genetic alterations also play an important role pathogenesis cervical cancer. Therefore, we have investigated several (onco)genes carcinoma. In tumors from 136 patients with stage I and II cancer cervix, expression epidermal growth factor receptor (EGFR), c-erbB-2/neu, p53, murine double minute 2 (MDM-2) was studied using immunohistochemistry. 32 cases, amplification EGFR, MDM-2, c-myc by Southern blot hybridization. levels these proteins were correlated HPV positivity, International Federation Gynecologists Obstetricians stage, lymph node metastases, tumor diameter, vessel invasion, disease-free overall survival. Moderate/strong EGFR observed 54% tumors. c-erbB-2/neu focally positive 12 cases. p53 showed moderate/strong 32% Thirteen % a this to (P<0.001). Only associated reduced (P = 0.002) survival 0.003). multivariate analysis, association overexpression poor prognosis independent status. Gene found for (four cases), c-erbB-2/ neu (two (six cases). two tumors, rearrangement found, probably due integration HPV. conclusion, is predictor earlier stages (stage II) MDM-2 are each may interaction importance relatively small
aacrjournals.org参考文章(58)
Jiandong Chen, Jiayuh Lin, Arnold J. Levine, Regulation of transcription functions of the p53 tumor suppressor by the mdm-2 oncogene Molecular Medicine. ,vol. 1, pp. 142- 152 ,(1995) , 10.1007/BF03401562
Patricio Gariglio, Miguel Cruz, Rodolfo Ocadiz, Ana M. Graef, Rene Sauceda, High Correlation between Molecular Alterations of the c-myc Oncogene and Carcinoma of the Uterine Cervix Cancer Research. ,vol. 47, pp. 4173- 4177 ,(1987)
S.T. Cole, O. Danos, Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products. Journal of Molecular Biology. ,vol. 193, pp. 599- 608 ,(1987) , 10.1016/0022-2836(87)90343-3
M S Greenblatt, W P Bennett, M Hollstein, C C Harris, Mutations in the p53 Tumor Suppressor Gene: Clues to Cancer Etiology and Molecular Pathogenesis Cancer Research. ,vol. 54, pp. 4855- 4878 ,(1994)
Catherine E. Greer, Sandra L. Peterson, Nancy B. Kiviat, M. Michele Manos, PCR amplification from paraffin-embedded tissues. Effects of fixative and fixation time. American Journal of Clinical Pathology. ,vol. 95, pp. 117- 124 ,(1991) , 10.1093/AJCP/95.2.117
G. R. Taylor, M. J. McPherson, B. D. Hames, PCR 2 : a practical approach IRL Press at Oxford University Press. ,(1995)
William T. Creasman, New gynecologic cancer staging Gynecologic Oncology. ,vol. 58, pp. 157- 158 ,(1995) , 10.1006/GYNO.1995.1203
Hans Ikenberg, Klaus Matthay, Beate Schmitt, Thomas Bauknecht, Marion Kiechle-Schwarz, Axel Göppinger, Albrecht Pfleiderer, p53 mutation and MDM2 amplification are rare even in human papillomavirus-negative cervical carcinomas. Cancer. ,vol. 76, pp. 57- 66 ,(1995) , 10.1002/1097-0142(19950701)76:1<57::AID-CNCR2820760108>3.0.CO;2-4
Peter Devilee, Marianne van den Broek, Nel Kuipers-Dukshoorn, Rukmini Kolluri, P.Meera Khan, Peter L. Pearson, Cees J. Cornelisse, At least four different chromosomal regions are involved in loss of heterozygosity in human breast carcinoma Genomics. ,vol. 5, pp. 554- 560 ,(1989) , 10.1016/0888-7543(89)90023-2
Kong-Bung Ohoo, Kowit Yu Chong, Absence of mutation in the p53 and the retinoblastoma susceptibility genes in primary cervical carcinomas. Virology. ,vol. 193, pp. 1042- 1046 ,(1993) , 10.1006/VIRO.1993.1224