High-yield continuous production of nicotinic acid via nitrile hydratase-amidase cascade reactions using cascade CSMRs.
作者： L. Cantarella , A. Gallifuoco , A. Malandra , L. Martínková , A. Spera
DOI: 10.1016/J.ENZMICTEC.2010.12.010
关键词: Nitrile hydratase 、 Combinatorial chemistry 、 Nitrile 、 Amidase 、 Yield (chemistry) 、 Bioconversion 、 Cascade reaction 、 Substrate (chemistry) 、 Reaction rate 、 Organic chemistry 、 Chemistry
摘要: High yields of nicotinic acid from 3-cyanopyridine bioconversion were obtained by exploiting the in situ nitrile hydratase-amidase enzymatic cascade system Microbacterium imperiale CBS 498-74. Experiments carried out continuously stirred tank UF-membrane bioreactors (CSMRs) arranged series. This reactor configuration enables both enzymes, involved reaction, to work with optimized kinetics, without any purification, their differing temperature dependences. To this end, first CSMR, for properties NHase, was operated (i) at low (5°C), limiting inactivation more fragile enzyme, hydratase, (ii) a high residence time (24 h) overcome reaction rate limitation. The second AMase, higher (50°C), lower (6h), and (iii) substrate (3-cyanopyridine) concentration control excess inhibition. appropriate choice operational conditions enabled total conversion 3-cyanpyridine (up 200 mM) into be achieved steady-state long periods. Higher concentrations required two CSMRs NHase series drive completion.
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