Catalase and superoxide dismutase gene expression and distribution during stem development in maize

摘要: The temporal and spatial patterns of expression the Cat Sod genes encoding multiple catalases superoxide dismutases in maize have been examined throughout stem development. Three stages development defined based upon catalase activity profiles internode elongation. At stage 1, is low internodes remain short; at 2, dramatically increases rapidly elongate; 3, decreases to levels intermediate 1 elongation ceases. Zymogram analysis immunoassays show that only CAT-3 isozyme present stem, even though both Cat1 Cat3 mRNAs accumulate Cat2 mRNA not detectable developing stem. In full-grown stems localized primarily sclerenchyma beneath epidermis around vascular bundles may possibly play a role lignification. Unlike catalase, all dismutase (SOD) isozymes transcripts are Thus, these two major antioxidant gene-enzyme systems differential during maize. © 1992 Wiley-Liss, Inc.