Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells
作者： Sergio Bernardini , Stefano Cianfarani , Anna Spagnoli , Margherita Annicchiarico-Petruzzelli , Gerry Melino
DOI: 10.1111/J.1365-2826.1994.TB00601.X
关键词: Retinoic acid 、 Internal medicine 、 Endocrinology 、 Paracrine signalling 、 Insulin-like growth factor 2 、 Cell culture 、 Autocrine signalling 、 Cellular differentiation 、 Insulin-like growth factor-binding protein 、 Biology 、 Binding protein
摘要: Insulin-like growth factors (IGFs) regulate the autocrine/paracrine of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), presence IGFBPs and possible modulation retinoic acid (RA), IGF-I, IGF-II truncated Des(1-3)IGF-I in conditioned medium (CM) human neuroblastoma SK-N-BE(2) cell line. demonstrated two IGFBPs, with MW 37 kDa 25 kDa. Following immunoprecipitation, they turned out be IGFBP-2 -4, respectively. RA-induced differentiation cells was accompanied a marked reduction intensity both IGFBP bands after 48 h (32% 24% control, respectively) 72 (2% 0% incubation. addition exogenous IGFs, did not induce differentiation, change pattern significantly, except for form induced decrease incubation (45% 18% respectively). These findings suggest that have role cells.
elsevier.com
sci-hub.se 参考文章(25)
Gerry Melino, Carol J. Thiele, Richard A. Knight, New insight on the biology of neuroectodermal tumors. Workshop report from the University of Rome Tor Vergata and the IDI-IRCCS on the genetics and control of growth, differentiation, and programmed cell death. Cancer Research. ,vol. 53, pp. 926- 928 ,(1993)
W H Busby, D G Klapper, D R Clemmons, Purification of a 31,000-dalton insulin-like growth factor binding protein from human amniotic fluid. Isolation of two forms with different biologic actions Journal of Biological Chemistry. ,vol. 263, pp. 14203- 14210 ,(1988) , 10.1016/S0021-9258(18)68206-7
Michel Binoux, Paul Hossenlopp, Danielle Seurin, Berta Segovia-Quinson, Sylvie Hardouin, Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the method for titration of the binding proteins and competitive binding studies. Analytical Biochemistry. ,vol. 154, pp. 138- 143 ,(1986) , 10.1016/0003-2697(86)90507-5
P Cohen, H C Graves, D M Peehl, M Kamarei, L C Giudice, R G Rosenfeld, Prostate-specific antigen (PSA) is an insulin-like growth factor binding protein-3 protease found in seminal plasma. The Journal of Clinical Endocrinology and Metabolism. ,vol. 75, pp. 1046- 1053 ,(1992) , 10.1210/JCEM.75.4.1383255
O M El-Badry, L J Helman, J Chatten, S M Steinberg, A E Evans, M A Israel, Insulin-like growth factor II-mediated proliferation of human neuroblastoma. Journal of Clinical Investigation. ,vol. 87, pp. 648- 657 ,(1991) , 10.1172/JCI115042
S. Cianfarani, V. J. Frost, M. O. Savage, J. M. P. Holly, Glucose does not influence the insulin-like growth factor (IGF) binding to carrier proteins (IGFBPs): Analysis of rat and human serum by western ligand blotting Cellular and Molecular Life Sciences. ,vol. 49, pp. 699- 701 ,(1993) , 10.1007/BF01923954
Shunichi Shimasaki, Nicholas Ling, Identification and molecular characterization of insulin-like growth factor binding proteins (IGFBP-1, -2, -3, -4, -5 and -6). Progress in Growth Factor Research. ,vol. 3, pp. 243- 266 ,(1991) , 10.1016/0955-2235(91)90003-M
K Matsumoto, C Gaetano, W H Daughaday, C J Thiele, Retinoic acid regulates insulin-like growth factor II expression in a neuroblastoma cell line. Endocrinology. ,vol. 130, pp. 3669- 3676 ,(1992) , 10.1210/ENDO.130.6.1375906
J Zapf, C Schmid, H P Guler, M Waldvogel, C Hauri, E Futo, P Hossenlopp, M Binoux, E R Froesch, Regulation of binding proteins for insulin-like growth factors (IGF) in humans. Increased expression of IGF binding protein 2 during IGF I treatment of healthy adults and in patients with extrapancreatic tumor hypoglycemia. Journal of Clinical Investigation. ,vol. 86, pp. 952- 961 ,(1990) , 10.1172/JCI114797
S E Gargosky, H M Pham, K F Wilson, F Liu, L C Giudice, R G Rosenfeld, Measurement and characterization of insulin-like growth factor binding protein-3 in human biological fluids: discrepancies between radioimmunoassay and ligand blotting Endocrinology. ,vol. 131, pp. 3051- 3060 ,(1992) , 10.1210/ENDO.131.6.1280211