Glucose does not influence the insulin-like growth factor (IGF) binding to carrier proteins (IGFBPs): Analysis of rat and human serum by western ligand blotting
作者： S. Cianfarani , V. J. Frost , M. O. Savage , J. M. P. Holly
DOI: 10.1007/BF01923954
关键词:
摘要: The insulin-like growth factors (IGFs) circulate bound to specific proteins (termed IGFBP-1 through IGFBP-6) that modulate IGF bioactivity in tissues. aim of this study was analyse the effects glucose on binding IGFBPs rat and human serum by means western ligand blotting. Serum samples were incubated with increasing concentrations (0 50 mmol/l), EDTA (25 mmol/l) added inhibit protease activity. To effect protection from activity, pregnant women (reported be very rich proteolytic activity against IGFBPs) previously glucose. Glucose did not affect 125I-IGF IGFBPs. intensity IGFBP-3 bands decreased considerably during incubation. This appeared due endogenous since decrease blocked addition EDTA. incubation produced a marked attenuation disappearance IGFBP-4 bands. In conclusion, our shows does influence either or serum, confirms presence normal non-pregnant demonstrates has no protective action
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Michel Binoux, Paul Hossenlopp, Danielle Seurin, Berta Segovia-Quinson, Sylvie Hardouin, Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the method for titration of the binding proteins and competitive binding studies. Analytical Biochemistry. ,vol. 154, pp. 138- 143 ,(1986) , 10.1016/0003-2697(86)90507-5
LINDA C. GIUDICE, ELEANOR M. FARRELL, HUNG PHAM, GEORGE LAMSON, RON G. ROSENFELD, Insulin-like growth factor binding proteins in maternal serum throughout gestation and in the puerperium: effects of a pregnancy-associated serum protease activity. The Journal of Clinical Endocrinology and Metabolism. ,vol. 71, pp. 806- 816 ,(1990) , 10.1210/JCEM-71-4-806
S. C. Davies, J. A. H. Wass, R. J. M. Ross, A. M. Cotterill, C. R. Buchanan, V. J. Coulson, J. M. P. Holly, The induction of a specific protease for insulin-like growth factor binding protein-3 in the circulation during severe illness. Journal of Endocrinology. ,vol. 130, pp. 469- 473 ,(1991) , 10.1677/JOE.0.1300469
A. M. TAYLOR, D. B. DUNGER, M. A. PREECE, J. M. P HOLLY, C. P. SMITH, J. A. H. WASS, S. PATEL, V. E. TATE, The growth hormone independent insulin-like growth factor-I binding protein BP-28 is associated with serum insulin-like growth factor-I inhibitory bioactivity in adolescent insulin-dependent diabetics. Clinical Endocrinology. ,vol. 32, pp. 229- 239 ,(1990) , 10.1111/J.1365-2265.1990.TB00859.X
L. S. Phillips, H. S. Young, Nutrition and Somatomedin: II. Serum Somatomedin Activity and Cartilage Growth Activity in Streptozotocin-diabetic Rats Diabetes. ,vol. 25, pp. 516- 527 ,(1976) , 10.2337/DIAB.25.6.516
ANNE-MARIA SUIKKARI, VEIKKO A. KOIVISTO, EEVA-MARJA RUTANEN, HANNELE YKI-JÄRVINEN, SIRKKA-LIISA KARONEN, MARKKU SEPPÄLÄ, Insulin Regulates the Serum Levels of Low Molecular Weight Insulin-Like Growth Factor-Binding Protein* The Journal of Clinical Endocrinology and Metabolism. ,vol. 66, pp. 266- 272 ,(1988) , 10.1210/JCEM-66-2-266
J R Baker, P A Metcalf, I M Holdaway, R N Johnson, Serum fructosamine concentration as measure of blood glucose control in type I (insulin dependent) diabetes mellitus. BMJ. ,vol. 290, pp. 352- 355 ,(1985) , 10.1136/BMJ.290.6465.352
S. Cianfarani, J. M. P. Holly, Somatomedin-binding proteins: what role do they play in the growth process? European Journal of Pediatrics. ,vol. 149, pp. 76- 79 ,(1989) , 10.1007/BF01995850
A. M. Cortizo, J. J. Gagliardino, Protein glycation: its role in the changes induced by diabetes in the properties of the serum insulin-like growth factor-I binding proteins. Journal of Endocrinology. ,vol. 131, pp. 33- 38 ,(1991) , 10.1677/JOE.0.1310033
GEORGE LAMSON, LINDA C. GIUDICE, RON G. ROSENFELD, A simple assay for proteolysis of IGFBP-3 The Journal of Clinical Endocrinology and Metabolism. ,vol. 72, pp. 1391- 1393 ,(1991) , 10.1210/JCEM-72-6-1391