Mechanisms and regulation of DNA end resection

摘要: DNA double-strand breaks (DSBs) are highly hazardous for genome integrity, because failure to repair these lesions can lead genomic instability. DSBs arise accidentally at unpredictable locations into the genome, but they also normal intermediates in meiotic recombination. Moreover, natural ends of linear chromosomes resemble DSBs. Although intrachromosomal potent stimulators damage response, packaged protective structures called telomeres that suppress repair/recombination activities. and functionally different, both undergo 5′–3′ nucleolytic degradation ends, a process known as resection. The resulting 3′-single-stranded overhangs enable by homologous recombination (HR), whereas allow action telomerase telomeres. molecular activities required DSB telomere end resection similar, indicating initial steps HR telomerase-mediated elongation related. Resection must be tightly regulated time space ensure stability cell survival.