SERBP1 affects homologous recombination-mediated DNA repair by regulation of CtIP translation during S phase

摘要: DNA double-strand breaks (DSBs) are the most severe type of damage and primarily repaired by non-homologous end joining (NHEJ) homologous recombination (HR) in G1 S/G2 phase, respectively. Although CtBP-interacting protein (CtIP) is crucial resection during HR following DSBs, little known about how CtIP levels increase an S phase-specific manner. Here, we show that Serpine mRNA binding 1 (SERBP1) regulates expression at translational level phase. In response to camptothecin-mediated CHK1 RPA2 phosphorylation, which hallmarks activation, was abrogated SERBP1-depleted cells. We identified as a target SERBP1 using RNA immunoprecipitation-coupled sequencing, confirmed depletion resulted reduction polysome-associated concomitant loss These effects were reversed reconstituting cells with wild-type SERBP1, but not ΔRGG, defective mutant, suggesting regulation translation association mRNA. results indicate affects HR-mediated repair DSBs