Protein composition of Cucurbita maxima and C. moschata seeds
作者： H. A. Castro , M. J. Galvez , S. R. Gonzalez , C. B. Villamil
DOI: 10.1007/S10535-006-0015-3
关键词: Ouchterlony double immunodiffusion 、 Polyacrylamide gel electrophoresis 、 Interspecific hybrids 、 Jaccard index 、 Chromatography 、 Protein composition 、 Cucurbita maxima 、 Biology 、 Plant science 、 Horticulture
摘要: Seeds of Cucurbita maxima, C. moschata and their interspecific hybrids were used to evaluate the intrapopulational interpopulational variation protein composition. Three immunoprecipitating systems common all studied samples detected by Ouchterlony technique. Fourteen bands identified polyacrylamide gel electrophoresis (PAGE) whereas 23 sodiumdodecylsulfate (SDS)-PAGE. Using Western blotting (WB) also detected. The Jaccard's index similarity calculated from SDS-PAGE WB varied between 91 100 % for compared pairs samples. These results demonstrate a high uniformity in composition do not allow clear characterization.
springer.com
sci-hub.se 参考文章(30)
Thomas Spector, Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein. Analytical Biochemistry. ,vol. 86, pp. 142- 146 ,(1978) , 10.1016/0003-2697(78)90327-5
S.S. Jha, D. Ohri, Comparative Study of Seed Protein Profiles in the Genus Pisum Biologia plantarum. ,vol. 45, pp. 529- 532 ,(2002) , 10.1023/A:1022316907401
D. E. Fairbrothers, Chemosystematics with emphasis on systematic serology. Chemosystematics with emphasis on systematic serology.. ,(1968)
William J. Kuhns, M. W. Chase, Specificity of serological reactions : Landsteiner centennial New York Academy of Sciences. ,(1970)
David M. Bates, Charles Jeffrey, Richard W. Robinson, Biology and Utilization of the Cucurbitaceae ,(1990)
Tod F. Stuessy, Plant taxonomy : the systematic evaluation of comparative data ,(1990)
Arthur Cronquist, The evolution and classification of flowering plants ,(1968)
A.S. Haider, A.R. El-Shanshoury, Variability of Storage Proteins and Esterase Isozymes in Vicia Sativa Subspecies Biologia plantarum. ,vol. 43, pp. 205- 209 ,(2000) , 10.1023/A:1002791824350
Hermann Schägger, Gebhard von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry. ,vol. 166, pp. 368- 379 ,(1987) , 10.1016/0003-2697(87)90587-2
V. H. Heywood, Flowering Plants of the World ,(1985)