Protein profiling in F1 and F2 generations of two tomato genotypes differing in ripening time

作者: G. R. Rodriguez , L. Sequin , G. R. Pratta , R. Zorzoli , L. A. Picardi

DOI: 10.1007/S10535-008-0107-3

关键词:

摘要: Pericarp polypeptide profiles were analyzed at three ripening stages in the F1 hybrid and F2 population from cross between accessions: LA1385 (Lycopersicon esculentum var. cerasiforme) 804627 (L. esculentum, a homozygous genotype for nor mutant). Six polymorphic polypeptides observed LA1385, while no among was 804627. On other hand, some not parents whereas others present both parental genotypes unnoticeable genotype. From cluster analysis on protein of population, differential expression proteins allowed to distinguish mature green (MG) stage two stages, breaker (BR) red ripe stage, genetic background more important forming groups. The could be associated with fruit morphology traits such as 72 kDa MG diameter, height mass 47 found BR shelf life.

参考文章(21)
Vincenzo Pirrotta, Sylvain Poux, Raffaella Melfi, Maxim Pilyugin, Assembly of Polycomb complexes and silencing mechanisms. Genetica. ,vol. 117, pp. 191- 197 ,(2003) , 10.1023/A:1022992011833
Santiago Bortolotti, Silvana B. Boggio, Luciana Delgado, Elena G. Orellano, Estela M. Valle, Different induction patterns of glutamate metabolizing enzymes in ripening fruits of the tomato mutant green flesh Physiologia Plantarum. ,vol. 119, pp. 384- 391 ,(2003) , 10.1034/J.1399-3054.2003.00184.X
G. R. Pratta, R. Zorzoli, L. A. Picardi, E. M. Valle, Variability for the in vitro culture response in tomato recombinant inbred lines Biologia plantarum. ,vol. 50, pp. 421- 424 ,(2006) , 10.1007/S10535-006-0060-Y
A. R. Schuelter, F. L. Finger, V. W. D. Casali, S. H. Brommonschenkel, W. C. Otoni, Inheritance and genetic linkage analysis of a firm-ripening tomato mutant Plant Breeding. ,vol. 121, pp. 338- 342 ,(2002) , 10.1046/J.1439-0523.2002.00719.X
Gustavo R. Rodríguez, Guillermo R. Pratta, Roxana Zorzoli, Liliana A. Picardi, Transgressive segregation for fruit quality traits in a cross between exotic and mutant genotypes of Lycopersicon New Zealand Journal of Crop and Horticultural Science. ,vol. 33, pp. 373- 379 ,(2005) , 10.1080/01140671.2005.9514373
K. B. Alpert, S. D. Tanksley, High-resolution mapping and isolation of a yeast artificial chromosome contig containing fw2.2: a major fruit weight quantitative trait locus in tomato. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 15503- 15507 ,(1996) , 10.1073/PNAS.93.26.15503
H. A. Castro, M. J. Galvez, S. R. Gonzalez, C. B. Villamil, Protein composition of Cucurbita maxima and C. moschata seeds Biologia plantarum. ,vol. 50, pp. 251- 256 ,(2006) , 10.1007/S10535-006-0015-3
Fabrizio Carbone, Daniele Pizzichini, Giovanni Giuliano, Carlo Rosati, Gaetano Perrotta, Comparative profiling of tomato fruits and leaves evidences a complex modulation of global transcript profiles Plant Science. ,vol. 169, pp. 165- 175 ,(2005) , 10.1016/J.PLANTSCI.2005.03.011
William J. Hurkman, Charlene K. Tanaka, Solubilization of Plant Membrane Proteins for Analysis by Two-Dimensional Gel Electrophoresis Plant Physiology. ,vol. 81, pp. 802- 806 ,(1986) , 10.1104/PP.81.3.802