Anti-idiotypic monoclonal antibodies reacting with idiotope on isolated-denatured chains of an anti-CD4 monoclonal antibody.
作者： Perosa F , Ferrone S , Dammacco F
DOI:
关键词: Virology 、 Immunoglobulin light chain 、 Idiotype 、 Monoclonal 、 Molecular biology 、 Anti-CD4 Monoclonal Antibody 、 Polyclonal antibodies 、 Monoclonal antibody 、 Idiotopes 、 Antibody 、 Biology
摘要: Hybridization of a non-secreting mouse myeloma cell line NSO with splenocytes from BALB/c hyperimmunized the anti-CD4 monoclonal antibody (mAb) HP2/6 generated anti-idiotypic (anti-id) mAb F11-2113, F11-2302 and F11-2444, which recognize idiotope(s) (id) that are same or spatially close to each other located outside antigen-combining site immunizing mAb. Binding inhibition assay showed id not expressed either on polyclonal immunoglobulins (Ig). Western blotting analysis defined by anti-id F11-2444 similarly separated heavy light chains suggested they likely be 'sequence-dependent' since their expression is conserved following SDS reducing reagents treatment. This finding unique inasmuch as have been described only auto-antibody immunoregulatory properties.
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