Phosphorylation of Caspase-9 by CDK1/Cyclin B1 Protects Mitotic Cells against Apoptosis
作者： Lindsey A. Allan , Paul R. Clarke
DOI: 10.1016/J.MOLCEL.2007.03.019
关键词: Mitosis 、 Cancer research 、 Cell cycle 、 Cyclin A 、 Phosphorylation 、 Cell biology 、 Apoptosis 、 Cyclin-dependent kinase 1 、 Biology 、 Cyclin B1 、 Cyclin B
摘要: Proliferating metazoan cells respond to damage that has the potential cause genomic instability by restricting cell division cycle or initiating apoptosis. The molecular mechanisms determining balance between these responses are not well understood. Here, we show apoptotic initiator protease caspase-9 is regulated during through periodic phosphorylation at an inhibitory site, Thr125. This site phosphorylated CDK1/cyclin B1 mitosis and in response microtubule poisons arrest this stage of cycle. Using RNA interference strategy, induction apoptosis from drugs dependent greatly increased when endogenous replaced a nonphosphorylatable mutant. Thus, Thr125 sets threshold for activation intrinsic pathway cycle, restrains mitosis, determines sensitivity antimitotic drugs.
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Kwan-Hyuck Baek, Hyun-Jin Shin, Sook-Jung Jeong, Joong-Won Park, Frank McKeon, Chang-Woo Lee, Chang-Min Kim, Caspases-dependent cleavage of mitotic checkpoint proteins in response to microtubule inhibitor. Oncology Research. ,vol. 15, pp. 161- 168 ,(2005) , 10.3727/096504005776367906
L. A. Groom, A. A. Sneddon, D. R. Alessi, S. Dowd, S. M. Keyse, Differential regulation of the MAP, SAP and RK/p38 kinases by Pyst1, a novel cytosolic dual-specificity phosphatase. The EMBO Journal. ,vol. 15, pp. 3621- 3632 ,(1996) , 10.1002/J.1460-2075.1996.TB00731.X
Michael B. Kastan, Jiri Bartek, Cell-cycle checkpoints and cancer Nature. ,vol. 432, pp. 316- 323 ,(2004) , 10.1038/NATURE03097
Lindsey A. Allan, Nick Morrice, Suzanne Brady, Gareth Magee, Shalini Pathak, Paul R. Clarke, Inhibition of caspase-9 through phosphorylation at Thr 125 by ERK MAPK Nature Cell Biology. ,vol. 5, pp. 647- 654 ,(2003) , 10.1038/NCB1005
Leland Hartwell, Defects in a cell cycle checkpoint may be responsible for the genomic instability of cancer cells. Cell. ,vol. 71, pp. 543- 546 ,(1992) , 10.1016/0092-8674(92)90586-2
Keisuke Kuida, Tarik F Haydar, Chia-Yi Kuan, Yong Gu, Choji Taya, Hajime Karasuyama, Michael S.-S Su, Pasko Rakic, Richard A Flavell, Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9. Cell. ,vol. 94, pp. 325- 337 ,(1998) , 10.1016/S0092-8674(00)81476-2
Zachary T. Schafer, Sally Kornbluth, The Apoptosome: Physiological, Developmental, and Pathological Modes of Regulation Developmental Cell. ,vol. 10, pp. 549- 561 ,(2006) , 10.1016/J.DEVCEL.2006.04.008
Michael H Cardone, Natalie Roy, Henning R Stennicke, Guy S Salvesen, Thomas F Franke, Eric Stanbridge, Steven Frisch, John C Reed, Regulation of Cell Death Protease Caspase-9 by Phosphorylation Science. ,vol. 282, pp. 1318- 1321 ,(1998) , 10.1126/SCIENCE.282.5392.1318
Daniela A. Brito, Conly L. Rieder, Mitotic Checkpoint Slippage in Humans Occurs via Cyclin B Destruction in the Presence of an Active Checkpoint Current Biology. ,vol. 16, pp. 1194- 1200 ,(2006) , 10.1016/J.CUB.2006.04.043
Morag C. Martin, Lindsey A. Allan, Michelle Lickrish, Catherine Sampson, Nick Morrice, Paul R. Clarke, Protein Kinase A Regulates Caspase-9 Activation by Apaf-1 Downstream of Cytochrome c Journal of Biological Chemistry. ,vol. 280, pp. 15449- 15455 ,(2005) , 10.1074/JBC.M414325200