Relative V beta transcript levels in thymus and peripheral lymphoid tissues from various mouse strains. Inverse correlation of I-E and Mls expression with relative abundance of several V beta transcripts in peripheral lymphoid tissues.

摘要: We have measured the relative levels of transcripts for 15 22 known V beta gene segments. The level highest and lowest expressed segment differed by greater than 20-fold in thymus an even larger difference was observed periphery. expressions were unrelated to order genes on chromosome. For most segments, transcript same periphery, suggesting that thymic selection general does not act solely upon segment. One BALB B10 mice strains exception this rule. 5.2 expression periphery inversely correlated with MHC class II molecule I-E. Five segments had reduced Mls-1a compared their or found Mls-1b mice. peripheral 3 vary Mls-2 haplotypes. observation certain are when favors hypothesis self tolerance at T cell results elimination self-reactive cells, rather paralysis a block some post-transcriptional step. Finally, wide variability suggests mechanisms exist import early bias repertoire. Whether from differential rearrangement rates, events occurring after TCR-alpha/beta immature/nonmature thymocyte surfaces is yet be determined.