DEMONSTRATION OF MEMBRANE-BOUND RECEPTORS BY MEANS OF AUTORADIOGRAPHY
作者： VINCI MIZUHIRA , JUNKO YOKOFUJITA , HIDENORI SUZUKI , YOSHIE SUGIURA
DOI: 10.1267/AHC.19.687
关键词: Estrogen 、 Binding site 、 Biology 、 Cerebrum 、 Internal medicine 、 Molecular biology 、 Bungarotoxin 、 Endocrinology 、 Hormone 、 Cell surface receptor 、 Cerebellum 、 Receptor
摘要: The binding sites of membrane-bound receptors such as insulin, acetyl choline, concanavaline A (Con A) are clearly demonstrated with a radioactive isotope (hot RI) incubation after light fixation. This type autoradiography (ARG) visualizes the correct good morphological characteristics. For steroid hormones estrogen including vitamine D3, genomic receptor cannot be samples except essential specific strong affinity in special granules eosinophilic leucocytes. We 125I-insulin liver, red blood cells, and heart muscle; 125I-α-bungarotoxin (bungarotoxin) rat intercostal muscle motor end-plate, cerebellum, cerebrum; 125I- or 3H-Con on surface human cells; 3H-estradiol 3H-moxestrol leucocytes uterus.
elsevier.com
sci-hub.se 参考文章(10)
YOSHIE SUGIURA, VINCI MIZUHIRA, Strong affinity of estradiol and its ligands for eosinophilic leucocytes in the rat and mouse uteri during the estrous cycle Acta Histochemica Et Cytochemica. ,vol. 17, pp. 665- 680 ,(1984) , 10.1267/AHC.17.665
Yuji Ishikawa, Yutaka Shimada, Fluorescent staining of neuromuscular junctions by using the antibody against acetylcholine receptors of Narke japonica, and double staining with the antibody and erabutoxin b. Brain Research. ,vol. 224, pp. 45- 54 ,(1981) , 10.1016/0006-8993(81)91115-X
Vinci Mizuhira, Michiko Shiihashi, Yutaka Futaesaku, High-speed electron microscope autoradiographic studies of diffusible compounds. Journal of Histochemistry and Cytochemistry. ,vol. 29, pp. 143- 160 ,(1981) , 10.1177/29.1A_SUPPL.7288151
Yasuyoshi Arimatsu, Akiko Seto, Takehiko Amano, An atlas of α‐bungarotoxin binding sites and structures containing acetylcholinesterase in the mouse central nervous system The Journal of Comparative Neurology. ,vol. 198, pp. 603- 631 ,(1981) , 10.1002/CNE.901980405
Yasuyoshi Arimatsu, Akiko Seto, Takehiko Amano, Localization of α-bungarotoxin binding sites in mouse brain by light and electron microscopic autoradiography Brain Research. ,vol. 147, pp. 165- 169 ,(1978) , 10.1016/0006-8993(78)90782-5
J P Bourgeois, J L Popot, A Ryter, J P Changeux, Quantitative studies on the localization of the cholinergic receptor protein in the normal and denervated electroplaque from Electrophorus electricus. Journal of Cell Biology. ,vol. 79, pp. 200- 216 ,(1978) , 10.1083/JCB.79.1.200
YASUYOSHI ARIMATSU, QUANTITATIVE LIGHT MICROSCOPIC AUTORADIOGRAPHY FOR α-BUNGAROTOXIN BINDING CAPACITY IN THE MOUSE BRAIN Acta Histochemica Et Cytochemica. ,vol. 15, pp. 600- 607 ,(1982) , 10.1267/AHC.15.600
HIDENORI SUZUKI, VINCI MIZUHIRA, Three-dimensional observation of the radioactive concanavalin a receptors on the surface of human red blood corpuscles utilizing scanning electron microscope autoradiography Acta Histochemica Et Cytochemica. ,vol. 15, pp. 624- 636 ,(1982) , 10.1267/AHC.15.624
YUJI ISHIKAWA, YUTAKA SHIMADA, STAINING OF ACETYLCHOLINE RECEPTORS AT THE ADULT MOUSE AND EMBRYONIC CHICK NEUROMUSCULAR JUNCTIONS USING ERABUTOXIN B AND THE ANTIBODY AGAINST ACETYLCHOLINE RECEPTORS Acta Histochemica Et Cytochemica. ,vol. 15, pp. 583- 591 ,(1982) , 10.1267/AHC.15.583
VINCI MIZUHIRA, MICHIKO SHIIHASHI, YOSHIE SUGIURA, Demonstration of receptor sites with electron microscope autoradiography —distribution of receptors for estradiol and its ligands in the mouse and rat uteri— Acta Histochemica Et Cytochemica. ,vol. 15, pp. 572- 582 ,(1982) , 10.1267/AHC.15.572