A genomic analysis of RNA polymerase II modification and chromatin architecture related to 3′ end RNA polyadenylation

摘要: Genomic analyses have been applied extensively to analyze the process of transcription initiation in mammalian cells, but less transcript 3′ end formation and termination. We used a novel approach prepare fragments from polyadenylated RNA, mapped position poly(A) addition site using oligonucleotide arrays tiling 1% human genome. This revealed more ends than had annotated. The distribution these relative RNA polymerase II (PolII) di- trimethylated lysine 4 36 histone H3 was compared. A substantial fraction unannotated are intronic antisense embedding gene. Poly(A) annotated messages lie on average 2 kb upstream PolII binding (termination). Near termination sites, some internal unphosphorylated C-terminal domain (CTD) serine phosphorylated (POLR2A) accumulate, suggesting pausing perhaps dephosphorylation prior release. Lysine trimethylation occurs across transcribed genes, sometimes alternating with stretches DNA which dimethylation is prominent. methylation decreases at or near polyadenylation, disappearing before disappearance release DNA. Our results suggest that loss 3 later polymerase. latter often associated pausing. Overall, our study reveals extensive sites provides insights into events occur during formation.