The Pu.1 locus is differentially regulated at the level of chromatin structure and noncoding transcription by alternate mechanisms at distinct developmental stages of hematopoiesis.

摘要: The Ets family transcription factor PU.1 is crucial for the regulation of hematopoietic development. Pu.1 activated in stem cells and expressed mast cells, B granulocytes, macrophages but switched off T cells. Many factors regulating have been identified, little known about how they organize chromatin We analyzed promoter upstream regulatory element (URE) using vivo footprinting immunoprecipitation assays. In was bound by a set different from that myeloid adopted alternative architectures. at URE open same binding sites were occupied as RUNX1 to precursor down-regulated maturing knockout Fli-1 compensated lack PU.1, both proteins could occupy subset cis elements PU.1-expressing addition, we identified novel URE-derived noncoding transcripts subject tissue-specific regulation. Our results provide important insights into overlapping, different, sets program structures system.