IL-4 and IL-13 production in differentiated T helper type 2 cells is not IL-4 dependent.

摘要: CD4+ T cell differentiation into cells capable of producing IL-4 and IL-13 (Th2 cells) requires the presence is STAT-6 dependent. Here we show that not required for or production by Th2 cells. Anti-IL-4 anti-IL-4R Ab did diminish in response to TCR-mediated stimulation, nor enhance stimulation with limiting amounts Ag. prepared from knockout mice were mRNA immobilized anti-CD3. increase expression. Despite failure effect primed cells, a binding element was demonstrated promoter. The authenticity this oligonucleotide competition, supershifting anti-STAT-6 Ab, IL-4-inducible effects on transcription reporter gene under control multimerized fused an minimal Nonetheless, promoter construct lacking as effective containing anti-CD3-induced transcription. Thus, element, if biologically active, must function at step responsiveness distinct acute Ag