Cell cycle regulation of p34cdc2 kinase activity in Physarum polycephalum.

摘要: The regulation of the mitotic histone H1 kinase activity has been analyzed during naturally synchronous cell cycle Physarum polycephalum plasmodia. universal binding property p13suc1 Schizosaccharomyces pombe gene product was used to precipitate and assay cdc2 activity. peaks at beginning metaphase its decline, which requires protein synthesis, appears be an early event process. Microtubular poisons, temperature shifts DNA synthesis inhibitors were perturb regulatory pathways characterize their effects on activation. Our results suggest that full activation least two successive triggering signals involving microtubular components synthesis.