Enzymatic hydrolysis of cellulose to glucose lung immobilized ?-glucosidase

摘要: The production of sugars by enzymic hydrolysis cellulose is a multistep process which includes conversion the intermediate cellobiose to glucose B-glucosidase. Aside from its role as an inhibits endolglucanase components typical cellulase enzyme systems. Because these systems often contain insufficient concentrations B-glucosidase prevent accumulation inhibitory cellobiose, use supplemental immobilized increase yields was investigated. Immobolized Aspergillus phoenicis produced sorption on controlled-pore alumina with approximately 90% activity retention. product lost only 10% original during on-stream reaction period 500 h substrate. Maximum occurred near pH 3.5, and apparent activation energy approx. 11 kcal/mol. used together Trichoderma reesei hydrolyze cellulosic materials, such Solka Floc, corn stover, exploded wood. Increased greater were observed when contained