Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity.

摘要: Summary Plant–pathogen interactions are complex associations driven by the interplay of host and microbe-encoded factors. With secreted pathogen proteins (effectors) immune signalling components found in plant nucleus, this compartment is a battleground where susceptibility specified. We hypothesized that, defining changes nuclear proteome during infection, we can pinpoint vital required for immunity or susceptibility. We tested hypothesis documenting dynamic tomato (Solanum lycopersicum) infection oomycete Phytophthora capsici. enriched nuclei from infected noninfected tissues quantitatively assessed proteome. then role candidate regulators through functional assays. We demonstrated that dynamically P. capsici infection. observed known factors were differentially expressed and, based on observation, selected set successfully implicated to P. capsici. Our work exemplifies powerful strategy gain rapid insight into important processes underpin crop traits such as resistance. have identified large may pathogens crops.