Surface plasmon resonance analysis of staphylococcal enterotoxin B in food.
作者： AVRAHAM RASOOLY
DOI: 10.4315/0362-028X-64.1.37
关键词: Biosensor 、 Spr biosensor 、 Matrix (chemical analysis) 、 Antigen 、 Chemistry 、 Antibody 、 Microbiology 、 Surface plasmon resonance 、 Enterotoxin 、 Chromatography
摘要: Surface plasmon resonance (SPR) biosensors are electro-optical instruments used for analyzing real-time protein-protein interactions. This work evaluates an SPR biosensor (Biacore 3000) in the detection of staphylococcal enterotoxin B (SEB) foods. A sandwich immunosensor involving two antibodies was used. The capturing antibody, bound covalently to surface chip, performs initial binding antigen and a second antibody binds captured antigen. makes verification possible amplifies signal. Pure SEB as well spiked foods (milk meat) were detected with little interference from food matrix. In control experiments uncontaminated samples no significant signal detected. assay detects at ~10 ng/ml rapidly, within 2 min. entire measurement cycle (including washing chip regeneration) may take 5 min using one or 8 antibodies. These ...
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