1,2-α-l-Fucosynthase: A glycosynthase derived from an inverting α-glycosidase with an unusual reaction mechanism
作者： Jun Wada , Yuji Honda , Masamichi Nagae , Ryuichi Kato , Soichi Wakatsuki
DOI: 10.1016/J.FEBSLET.2008.09.054
关键词: Hydrolysis 、 Chemistry 、 Reaction mechanism 、 Mutant 、 N-Acetylglucosamine 、 Protein structure 、 Glycosynthase 、 Glycoside hydrolase 、 Stereochemistry 、 Enzyme 、 Biophysics 、 Genetics 、 Cell biology 、 Biochemistry 、 Molecular biology 、 Structural biology
摘要: Fucosyloligosaccharides have great therapeutic potential. Here we present a new route for synthesizing Fucα1,2Gal linkage by introducing glycosynthase technology into 1,2-α-l-fucosidase. The enzyme adopts unique reaction mechanism, in which asparagine-423 activated aspartic acid-766 acts as base while asparagine-421 fixes both catalytic water and glutamic acid-566 (an acid) the proper orientations. Glycosynthase activity of N421G, N423G, D766G mutants was examined using β-fucosyl fluoride lactose, among them, mutant most effectively synthesized 2′-fucosyllactose. 1,2-α-l-Fucosynthase is first derived from an inverting α-glycosidase glycosidase with unusual mechanism.
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