Glycosyl fluorides in enzymatic reactions.

摘要: Glycosyl fluorides have considerable importance as substrates and inhibitors in enzymatic reactions. Their good combination of stability reactivity has enabled their use glycosyl donors with a variety carbohydrate processing enzymes. Moreover, the installation fluorine elsewhere on scaffold commonly modifies properties fluoride such that resultant compounds act slow or even enzyme action. This review covers for wild-type mutant glycosidases other enzymes catalyze transfer. The substituted transfer tools investigation mechanism is discussed, including labeling active site residues. Synthetic applications which are used transglycosylation reactions synthesis oligo- polysaccharides then covered, glycosidases, so-called glycosynthases, able to formation glycosides without competing hydrolysis. Finally, short overview phosphorylases phosphoglucomutase given.