miR-17 overexpression in cystic fibrosis airway epithelial cells decreases interleukin-8 production

摘要: Interleukin (IL)-8 levels are higher than normal in cystic fibrosis (CF) airways, causing neutrophil infiltration and non-resolving inflammation. Overexpression of microRNAs that target IL-8 expression airway epithelial cells may represent a therapeutic strategy for fibrosis. protein mRNA were measured non-cystic bronchoalveolar lavage fluid bronchial brushings (n=20 per group). miRNAs decreased the lung predicted to quantified βENaC-transgenic, transmembrane conductance regulator (Cftr)-/- wild-type mice, primary range versus cell lines or stimulated with lipopolysaccharide, Pseudomonas-conditioned medium fluid. The effect miRNA overexpression on production was measured. miR-17 regulates its adult brushings, βENaC-transgenic mice chronically medium. inhibited basal agonist-induced F508del-CFTR homozygous CFTE29o(-) tracheal, CFBE41o(-) and/or IB3 cells. These results implicate defective CFTR, inflammation, neutrophilia mucus overproduction regulation miR-17. Modulating be novel anti-inflammatory other chronic inflammatory diseases.