Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease

摘要: Urolithiasis is a complex and multifactorial disorder characterized by the presence of stones in urinary tract. Urea cycle an important process involved disease progression. L-ornithine key amino acid urea converted to putrescine ornithine decarboxylase (ODC). Putrescine, spermidine spermine are natural polyamines that catabolized specific enzyme, spermidine/spermine acetyltransferase (SSAT). The single-nucleotide polymorphisms (SNPs) intron region ODC (+316 G>A) promoter SSAT (−1415 T>C) genes have been found be associated with expression levels. aim this study was examine whether 1 gene polymorphism SAT-1 potential genetic markers for susceptibility urolithiasis. A control group 104 healthy subjects 65 patients recurrent idiopathic calcium oxalate stone were enrolled into study. Polymerase chain reaction (PCR)-based restriction analysis performed PstI MspI enzyme digestion, respectively. genotype distribution studied −1415 T>C did not reveal significant difference between urolithiasis groups (P=0.713 0.853), Furthermore, no observed patient +316 G>A allele frequencies (P=0.877 0.644), In conclusion, results present suggest + 316 might risk factor