Affinity maturation by phage display.
作者： Holger Thie , Bernd Voedisch , Stefan Dübel , Michael Hust , Thomas Schirrmann
DOI: 10.1007/978-1-59745-554-1_16
关键词: Phage display 、 Recombinant DNA 、 Biochemistry 、 Antibody 、 Genomic library 、 Affinity maturation 、 Mutagenesis (molecular biology technique) 、 Peptide library 、 Chemistry 、 Panning (camera)
摘要: Antibodies are indispensable tools for research, diagnostics, and therapy. However, sometimes antibodies with the most favourable specificity profile lack sufficient affinity a desired application. Here, we describe method to increase of recombinant scFv antibody fragments based on random mutagenesis phage display under stringent conditions. Random mutations inserted by performing several rounds error-prone PCR. After construction mutated gene library, selection is performed panning washing conditions optimized off-rate-dependent selection. Alternatively, in solution competition can be used enrich binders improved binding properties.
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sci-hub.se 参考文章(20)
Michael Hust, André Frenzel, Thomas Schirrmann, Stefan Dübel, Selection of Recombinant Antibodies From Antibody Gene Libraries Methods of Molecular Biology. ,vol. 1101, pp. 305- 320 ,(2014) , 10.1007/978-1-62703-721-1_14
Kerrm Y.F. Yau, Ginette Dubuc, Shenghua Li, Tomoko Hirama, C. Roger MacKenzie, Lutz Jermutus, J. Christopher Hall, Jamshid Tanha, Affinity maturation of a VHH by mutational hotspot randomization Journal of Immunological Methods. ,vol. 297, pp. 213- 224 ,(2005) , 10.1016/J.JIM.2004.12.005
Daniele Sblattero, Andrew Bradbury, Exploiting recombination in single bacteria to make large phage antibody libraries. Nature Biotechnology. ,vol. 18, pp. 75- 80 ,(2000) , 10.1038/71958
Nigel M. Low, Philipp Holliger, Greg Winter, Mimicking Somatic Hypermutation: Affinity Maturation of Antibodies Displayed on Bacteriophage Using a Bacterial Mutator Strain Journal of Molecular Biology. ,vol. 260, pp. 359- 368 ,(1996) , 10.1006/JMBI.1996.0406
Gregory Coia, Peter J. Hudson, Robert A. Irving, Protein affinity maturation in vivo using E. coli mutator cells. Journal of Immunological Methods. ,vol. 251, pp. 187- 193 ,(2001) , 10.1016/S0022-1759(01)00300-3
Michael Hust, Stefan Dübel, Mating antibody phage display with proteomics Trends in Biotechnology. ,vol. 22, pp. 8- 14 ,(2004) , 10.1016/J.TIBTECH.2003.10.011
R. FINNERN, E. PEDROLLO, I. FISCH, J. WIESLANDER, J. D. MARKS, C. M. LOCKWOOD, W. H. OUWEHAND, Human autoimmune anti‐proteinase 3 scFv from a phage display library Clinical and Experimental Immunology. ,vol. 107, pp. 269- 281 ,(1997) , 10.1111/J.1365-2249.1997.254-CE1127.X
M. Kirsch, M. Zaman, D. Meier, S. Dübel, M. Hust, Parameters affecting the display of antibodies on phage Journal of Immunological Methods. ,vol. 301, pp. 173- 185 ,(2005) , 10.1016/J.JIM.2005.04.017
Steffen Goletz, Peter Astrup Christensen, Peter Kristensen, Dietmar Blohm, Ian Tomlinson, Greg Winter, Uwe Karsten, Selection of large diversities of antiidiotypic antibody fragments by phage display. Journal of Molecular Biology. ,vol. 315, pp. 1087- 1097 ,(2002) , 10.1006/JMBI.2001.5314
Robert Schier, Jacqueline Bye, Gerald Apell, Adrian McCall, Gregory P. Adams, Magnus Malmqvist, Louis M. Weiner, James D. Marks, Isolation of High-affinity Monomeric Human Anti-c-erbB-2 Single chain Fv Using Affinity-driven Selection Journal of Molecular Biology. ,vol. 255, pp. 28- 43 ,(1996) , 10.1006/JMBI.1996.0004