A new method to provide a fresh frozen prostate slice suitable for gene expression study and MR spectroscopy
作者： Helena Bertilsson , Anders Angelsen , Trond Viset , Haakon Skogseth , May-Britt Tessem
DOI: 10.1002/PROS.21260
关键词: Prostate 、 Biomedical engineering 、 RNA integrity number 、 Pathology 、 Prostatectomy 、 Cryopreservation 、 Gene expression 、 RNA 、 In vivo magnetic resonance spectroscopy 、 Prostate cancer 、 Medicine
摘要: Background Fresh frozen tissue from radical prostatectomy specimens is highly valuable material for research on gene expression and cellular metabolites. The purpose of this study was to develop a standardized method provide representative high quality sample without interfering with the routine histopathological procedure. Methods A complete transversal slice collected snap-frozen before formalin fixation processing remaining gland. freezing preserves original geometric shape, thus allowing subsampling specific cell populations thawing. RNA extracted 53 cylindrical subsamples (diameter 3 mm, thickness 2 mm) 16 consecutive slices. histological pattern evaluated by microscopy cryosection further analysis. Results Using novel harvesting close 400 slices have been collected. Whenever tumor present in both adjacent surrounding hematoxylin-eosin sections, we found cancer 88% showed very mean integrity number 9.16 (SD 0.53). MR spectra metabolic profiles containing several resonances, which deserve evaluation as possible biomarkers prostate cancer. After analysis still intact 8.40 1.53), makes it correlate transcriptomic metabolomic samples. Conclusion We safe procurement fresh slice, suitable spectroscopy.
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