The RNA Methyltransferase Dnmt2 Is Required for Efficient Dicer-2-Dependent siRNA Pathway Activity in Drosophila
作者： Zeljko Durdevic , Mehrpouya Balaghy Mobin , Katharina Hanna , Frank Lyko , Matthias Schaefer
DOI: 10.1016/J.CELREP.2013.07.046
关键词: RNA 、 Cell biology 、 Small interfering RNA 、 RNase P 、 Small RNA 、 Dicer 、 Transfer RNA 、 TRNA Methyltransferase 、 Gene expression 、 Biology 、 Genetics
摘要: Transfer RNA (tRNA) fragmentation in response to stress conditions has been described many organisms. tRNA fragments have found association with small interfering (siRNA) components, but the biological role of these interactions remains unclear. We report here that methyltransferase Dnmt2 is essential for efficient Dicer-2 (Dcr-2) function Drosophila. Using (sRNA) sequencing, we confirmed limits extent during heat-shock response. tRNAs as well serve Dcr-2 substrates, and degrades tRNA-derived sequences, especially under conditions. RNAs are able inhibit activity on long double-stranded (dsRNAs). Consequently, heat-shocked mutant animals accumulate dsRNAs, produce fewer siRNAs, show misregulation siRNA pathway-dependent genes. These results reveal impact pathways implicate modifications regulation sRNA homeostasis
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Rebecca L. Hurto, Unexpected Functions of tRNA and tRNA Processing Enzymes Advances in Experimental Medicine and Biology. ,vol. 722, pp. 137- 155 ,(2011) , 10.1007/978-1-4614-0332-6_9
Megha Ghildiyal, Hervé Seitz, Michael D Horwich, Chengjian Li, Tingting Du, Soohyun Lee, Jia Xu, Ellen LW Kittler, Maria L Zapp, Zhiping Weng, Phillip D Zamore, Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells. Science. ,vol. 320, pp. 1077- 1081 ,(2008) , 10.1126/SCIENCE.1157396
Elif Sarinay Cenik, Ryuya Fukunaga, Gang Lu, Robert Dutcher, Yeming Wang, Traci M. Tanaka Hall, Phillip D. Zamore, Phosphate and R2D2 Restrict the Substrate Specificity of Dicer-2, an ATP-Driven Ribonuclease Molecular Cell. ,vol. 42, pp. 172- 184 ,(2011) , 10.1016/J.MOLCEL.2011.03.002
Kuniaki Saito, Mikiko C. Siomi, Small RNA-mediated quiescence of transposable elements in animals. Developmental Cell. ,vol. 19, pp. 687- 697 ,(2010) , 10.1016/J.DEVCEL.2010.10.011
Mary Grace Goll, Finn Kirpekar, Keith A. Maggert, Jeffrey A. Yoder, Chih-Lin Hsieh, Xiaoyu Zhang, Kent G. Golic, Steven E. Jacobsen, Timothy H. Bestor, Methylation of tRNAAsp by the DNA Methyltransferase Homolog Dnmt2 Science. ,vol. 311, pp. 395- 398 ,(2006) , 10.1126/SCIENCE.1120976
Debrah M. Thompson, Roy Parker, Stressing Out over tRNA Cleavage Cell. ,vol. 138, pp. 215- 219 ,(2009) , 10.1016/J.CELL.2009.07.001
Francesca Tuorto, Reinhard Liebers, Tanja Musch, Matthias Schaefer, Sarah Hofmann, Stefanie Kellner, Michaela Frye, Mark Helm, Georg Stoecklin, Frank Lyko, None, RNA cytosine methylation by Dnmt2 and NSun2 promotes tRNA stability and protein synthesis. Nature Structural & Molecular Biology. ,vol. 19, pp. 900- 905 ,(2012) , 10.1038/NSMB.2357
Benjamin Czech, Colin D. Malone, Rui Zhou, Alexander Stark, Catherine Schlingeheyde, Monica Dus, Norbert Perrimon, Manolis Kellis, James A. Wohlschlegel, Ravi Sachidanandam, Gregory J. Hannon, Julius Brennecke, An endogenous small interfering RNA pathway in Drosophila Nature. ,vol. 453, pp. 798- 802 ,(2008) , 10.1038/NATURE07007
Katsutomo Okamura, Wei-Jen Chung, J. Graham Ruby, Huili Guo, David P. Bartel, Eric C. Lai, The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs. Nature. ,vol. 453, pp. 803- 806 ,(2008) , 10.1038/NATURE07015
Zeljko Durdevic, Matthias Schaefer, tRNA modifications: Necessary for correct tRNA-derived fragments during the recovery from stress? BioEssays. ,vol. 35, pp. 323- 327 ,(2013) , 10.1002/BIES.201200158