Evaluation of Multi-microbial Probiotics Produced by Submerged Liquid and Solid Substrate Fermentation Methods in Broilers

作者: Y. H. Shim , P. L. Shinde , J. Y. Choi , J. S. Kim , D. K. Seo

DOI: 10.5713/AJAS.2010.90446

关键词: Anaerobic bacteriaLactobacillus acidophilusWeight gainBiologyClostridiumColiform bacteriaFermentationFood scienceProbioticAntiinfective agent

摘要: Two experiments were conducted to evaluate multi-microbe submerged liquid (SLF) and solid substrate (SSF) fermented probiotic products in broilers. The SLF SSF probiotics comprised of Lactobacillus acidophilus (1.1×10 9 4×10 8 cfu/g), Bacillus subtilis 4.8×10 Saccharomyces cerevisiae (1.5×10 7 1.0×10 4 cfu/g) Aspergillus oryzae (2.6×10 4.3×10 respectively. In Exp. 1, 640 day-old Ross chicks allotted treatments, each comprising replicates (40 chicks/replicate). basal diet was prepared without any antimicrobials (negative control, NC), 20 mg/kg avilamycin (positive PC), 0.3% added the diets as treatments. Birds fed PC showed improved (p<0.001) overall weight gain F/G than birds NC diets; whereas, had better diet. Retention CP higher (p<0.05) PC, diets. tended have (p<0.10) cecal total anaerobic bacteria lesser coliforms noticed 2, randomly treatments a 2x2 factorial arrangement. Each treatment different (0.3% or SSF) with two antibiotics (10 colistin, avilamycin) used dietary greater (p<0.001), (p<0.05), retention energy protein Clostridium (d 35) colistin-supplemented less (p<0.01) when compared Additionally, colistin Thus, results this study suggest product by fermentation method be superior fermentation; moreover, feeding did not elicit interaction effect between antibiotic.

参考文章(38)
Steve Millam, Potato (Solanum tuberosum L.). Methods of Molecular Biology. ,vol. 344, pp. 25- 35 ,(2006) , 10.1385/1-59745-131-2:25
Seppo Salminen, Lactic acid bacteria as animal probiotics. Lactic acid bacteria.. pp. 315- 356 ,(2004) , 10.1201/9780824752033-25
D H Mowrey, L A Stobbs, J A d'Assonville, R H Wellenreiter, Effects of avilamycin on performance of broiler chickens. Veterinary Therapeutics. ,vol. 1, pp. 118- 124 ,(2000)
P. R. Ferket, T. P. Lyons, K. A. Jacques, Alternatives to antibiotics in poultry production: responses, practical experience and recommendations. Nutritional biotechnology in the feed and food industries. Proceedings of Alltech's 20th Annual Symposium: re-imagining the feed industry, Lexington, Kentucky, USA, 23-26 May 2004.. pp. 57- 67 ,(2004)
Paul A. Barrow, Probiotics for chickens Probiotics. pp. 225- 257 ,(1992) , 10.1007/978-94-011-2364-8_10
Stanford Moore, On the determination of cystine as cysteic acid. Journal of Biological Chemistry. ,vol. 238, pp. 235- 237 ,(1963) , 10.1016/S0021-9258(19)83985-6
Mary Ellen Sanders, Jos Huis in't Veld, Bringing a probiotic-containing functional food to the market: microbiological, product, regulatory and labeling issues Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 76, pp. 293- 315 ,(1999) , 10.1023/A:1002029204834
Jiankun Hu, Wenqing Lu, Chunlin Wang, Ronghua Zhu, Jiayun Qiao, Characteristics of Solid-state Fermented Feed and its Effects on Performance and Nutrient Digestibility in Growing-finishing Pigs Asian-australasian Journal of Animal Sciences. ,vol. 21, pp. 1635- 1641 ,(2008) , 10.5713/AJAS.2008.80032
K.-L. Chen, W.-L. Kho, S.-H. You, R.-H. Yeh, S.-W. Tang, C.-W. Hsieh, Effects of Bacillus subtilis var. natto and Saccharomyces cerevisiae mixed fermented feed on the enhanced growth performance of broilers Poultry Science. ,vol. 88, pp. 309- 315 ,(2009) , 10.3382/PS.2008-00224
G Weitnauer, A Mühlenweg, A Trefzer, D Hoffmeister, RD Süssmuth, G Jung, K Welzel, A Vente, U Girreser, A Bechthold, Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tü57 and production of new antibiotics Chemistry & Biology. ,vol. 8, pp. 569- 581 ,(2001) , 10.1016/S1074-5521(01)00040-0