Fast preparation of fungal DNA for PCR screening

作者: Kuang-Hung Liu , Yu-Ling Yeh , Wei-Chiang Shen

DOI: 10.1016/J.MIMET.2011.01.029

关键词: Fungal DNARapid dnaFungal geneticsYeastPolymerase chain reactionBiologyChemical fractionationBiochemistryDNAMicrobiology

摘要: Rapid DNA preparation for the quick screening is highly demanded in diverse research fields. Here, we combined an extraction buffer and heat treatment to generate templates from yeast filamentous fungal materials PCR. This method may be widely applicable species clinical basic studies.

参考文章(24)
Ying-Ku Lu, Kai-Hui Sun, Wei-Chiang Shen, Blue light negatively regulates the sexual filamentation via the Cwc1 and Cwc2 proteins in Cryptococcus neoformans Molecular Microbiology. ,vol. 56, pp. 480- 491 ,(2005) , 10.1111/J.1365-2958.2005.04549.X
John Pedersen, Don Liu, Rob Baird, Sue Coloe, Rapid mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology. ,vol. 38, pp. 471- 471 ,(2000) , 10.1128/JCM.38.1.471-471.2000
Philip Hieter, Mark D. Rose, Fred Winston, Methods in Yeast Genetics: A Laboratory Course Manual ,(1990)
J.L. Cenis, Rapid extraction of fungal DNA for PCR amplification Nucleic Acids Research. ,vol. 20, pp. 2380- 2380 ,(1992) , 10.1093/NAR/20.9.2380
Andrea Kotz, Johannes Wagener, Jakob Engel, Françoise Routier, Bernd Echtenacher, Andreas Pich, Manfred Rohde, Petra Hoffmann, Jürgen Heesemann, Frank Ebel, The mitA gene of Aspergillus fumigatus is required for mannosylation of inositol-phosphorylceramide, but is dispensable for pathogenicity Fungal Genetics and Biology. ,vol. 47, pp. 169- 178 ,(2010) , 10.1016/J.FGB.2009.10.001
Haruka Yamazaki, Norihiko Shiraishi, Kyoko Takeuchi, Yasuo Ohnishi, Sueharu Horinouchi, Characterization of alg2 encoding a mannosyltransferase in the zygomycete fungus Rhizomucor pusillus Gene. ,vol. 221, pp. 179- 184 ,(1998) , 10.1016/S0378-1119(98)00456-9
Frank Madeo, Eva Herker, Silke Wissing, Helmut Jungwirth, Tobias Eisenberg, Kai-Uwe Fröhlich, Apoptosis in yeast. Current Opinion in Microbiology. ,vol. 7, pp. 655- 660 ,(2004) , 10.1016/J.MIB.2004.10.012
Ikuko Miyajima, Masato Nakafuku, Naoki Nakayama, Charles Brenner, Atsushi Miyajima, Kozo Kaibuchi, Ken-ichi Arai, Yoshito Kaziro, Kunihiro Matsumoto, GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction Cell. ,vol. 50, pp. 1011- 1019 ,(1987) , 10.1016/0092-8674(87)90167-X