Real-time RT-PCR (TaqMan) assays for the detection of viruses associated with Rugose wood complex of grapevine.
作者： Fatima Osman , Adib Rowhani
DOI: 10.1016/J.JVIROMET.2008.09.005
关键词:
摘要: Real-time TaqMan® RT-PCR (TaqMan® RT-PCR) assays were developed to detect the viruses associated with Rugose wood complex of grapevines. The detected Rupestris stem pitting-associated virus in genus Foveavirus, Grapevine A, B and D Vitivirus. coat protein was found be most conserved gene within viral species, therefore, primers probes for designed from multiple alignment sequence various isolates each virus. Comparisons also made between conventional one step detection these using four-fold serial dilutions both purified RNA crude extract prepared grapevine tissue. Results showed that more sensitive could at 32- 256-fold higher extract, respectively, compared RT-PCR. use an internal control (18S rRNA) allowed comparison sample preparation protocols amplification efficiencies samples.
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sci-hub.se 参考文章(33)
P. Saldarelli, G. P. Martelli, A. Minafra, Vitivirus, a new genus of plant viruses. Archives of Virology. ,vol. 142, pp. 1929- 1932 ,(1997)
E. Choueiri, N. Abou-Ghanem, D. Bosca, Grapevine virus A and grapevine virus D are serologically distantly related Vitis: Journal of Grapevine Research. ,vol. 36, pp. 39- 41 ,(2015)
B. Rosciglione, P. Gugerli, Leaf roll and stem pitting diseases of grapevine: microscopic and serological analysis. Revue Suisse de Viticulture, d'Arboriculture et d'Horticulture. ,vol. 18, pp. 207- 211 ,(1986)
G. P. Martelli, Graft-transmissible diseases of grapevines: handbook for detection and diagnosis. Food and Agriculture Organization (FAO). ,(1993)
Pasquale Saldarelli, Adib Rowhani, Geoffrey Routh, Angelantonio Minafra, Michele Digiaro, Use of degenerate primers in a RT-PCR assay for the identification and analysis of some filamentous viruses, with special reference to clostero- and vitiviruses of the grapevine. European Journal of Plant Pathology. ,vol. 104, pp. 945- 950 ,(1998) , 10.1023/A:1008608506699
Maria Saponari, Keremane Manjunath, Raymond K Yokomi, Quantitative detection of Citrus tristeza virus in citrus and aphids by real-time reverse transcription-PCR (TaqMan). Journal of Virological Methods. ,vol. 147, pp. 43- 53 ,(2008) , 10.1016/J.JVIROMET.2007.07.026
M. Hren, J. Boben, A. Rotter, P. Kralj, K. Gruden, M. Ravnikar, Real‐time PCR detection systems for Flavescence dorée and Bois noir phytoplasmas in grapevine: comparison with conventional PCR detection and application in diagnostics Plant Pathology. ,vol. 56, pp. 785- 796 ,(2007) , 10.1111/J.1365-3059.2007.01688.X
D. A. G., R. C. Pearson, A. C. Goheen, Compendium of grape diseases. Mycologia. ,vol. 81, pp. 176- ,(1989) , 10.2307/3759482
Rick Mumford, Anna Skelton, Emily Metcalfe, Kathy Walsh, Neil Boonham, The reliable detection of Barley yellow and mild mosaic viruses using real-time PCR (TaqMan®) Journal of Virological Methods. ,vol. 117, pp. 153- 159 ,(2004) , 10.1016/J.JVIROMET.2004.01.006
A. Minafra, P. Saldarelli, G. P. Martelli, Grapevine virus A: nucleotide sequence, genome organization, and relationship in the Trichovirus genus Archives of Virology. ,vol. 142, pp. 417- 423 ,(1997) , 10.1007/S007050050088