Desempenho agronômico de videiras com e sem sintomas de viroses, e comparação molecular de isolados virais

作者: Monique Bezerra Nascimento , Thor Vinícius Martins Fajardo , Marcelo Eiras , Ana Beatriz Costa Czermainski , Osmar Nickel

DOI: 10.1590/S0100-204X2015000700004

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摘要: The objective of this work was to evaluate the effect viroses in symptomatic and asymptomatic grapevines on agronomic variables related plant vigor enological quality grapes, compare viral isolates obtained from these two conditions. Two experiments were performed with four cultivars. All plants indexed by real time, reverse transcription‑polymerase chain reaction (RT‑PCR) for probable occurrence following viruses: Grapevine virus A (GVA), B (GVB), D (GVD), leafroll‑associated (GLRaV‑1 ‑4, GLRaV‑4 strain 5), rupestris stem pitting‑associated (GRSPaV), fleck (GFkV). evaluated were: number buds burst not burst, branches or without bunches, total buds, fresh bunch weight, berry rachis berries per bunch, average soluble solids, titratable acidity, pH, pruned branch rootstock scion trunk diameters. negative effects more pronounced symptoms; however, it observed that symptoms also often infected. molecular analysis GRSPaV, GVA, GLRaV‑2, plants, resulted a high percentage nucleotide identities between homologous isolates.

参考文章(22)
G. P. Martelli, A. Minafra, A. Hadidi, Detection of grapevine closterovirus A in infected grapevine tissue by reverse transcription-polymerase chain reaction Vitis: Journal of Grapevine Research. ,vol. 31, pp. 221- 227 ,(2015)
N. Bertazzon, E. Angelini, ADVANCES IN THE DETECTION OF GRAPEVINE LEAFROLL-ASSOCIATED VIRUS 2 VARIANTS Journal of Plant Pathology. ,vol. 86, pp. 283- 290 ,(2004) , 10.4454/JPP.V86I4.999
Marcos Fernando Basso, Thor Vinícius Martins Fajardo, Marcelo Eiras, Ricardo Antônio Ayub, Osmar Nickel, Detecção e identificação molecular de vírus associados a videiras sintomáticas e assintomáticas Ciencia Rural. ,vol. 40, pp. 2249- 2255 ,(2010) , 10.1590/S0103-84782010001100001
C. Rosa, J. F. Jimenez, P. Margaria, A. Rowhani, Symptomatology and effects of viruses associated with rugose wood complex on the growth of four different rootstocks American Journal of Enology and Viticulture. ,vol. 62, pp. 207- 213 ,(2011) , 10.5344/AJEV.2011.10104
Marcos F. Basso, Thor V.M. Fajardo, Henrique P. Santos, Celito C. Guerra, Ricardo A. Ayub, Osmar Nickel, Fisiologia foliar e qualidade enológica da uva em videiras infectadas por vírus Tropical Plant Pathology. ,vol. 35, pp. 351- 359 ,(2010) , 10.1590/S1982-56762010000600003
Rayapati Naidu, Adib Rowhani, Marc Fuchs, Deborah Golino, Giovanni P. Martelli, Grapevine Leafroll: A Complex Viral Disease Affecting a High-Value Fruit Crop Plant Disease. ,vol. 98, pp. 1172- 1185 ,(2014) , 10.1094/PDIS-08-13-0880-FE
Aricléia de Moraes Catarino, Thor Vinícius Martins Fajardo, Gilvan Pio-Ribeiro, Marcelo Eiras, Osmar Nickel, Incidência de vírus em videiras no Nordeste brasileiro e caracterização molecular parcial de isolados virais locais Ciencia Rural. ,vol. 45, pp. 379- 385 ,(2015) , 10.1590/0103-8478CR20140587
Thor V.M. Fajardo, Marcelo Eiras, Henrique P. Santos, Osmar Nickel, Gilmar B. Kuhn, Detecção e caracterização biológica e molecular de Rupestris stem-pitting associated virus e seu efeito na fotossíntese de videiras Fitopatologia Brasileira. ,vol. 29, pp. 209- 214 ,(2004) , 10.1590/S0100-41582004000200016