Field Assessment and Diagnostic Methods for Detection of Grapevine Viruses
作者： Giorgio Gambino , Elisa Angelini , Ivana Gribaudo
DOI: 10.1007/978-90-481-9283-0_14
关键词:
摘要: The diagnosis of grapevine viral diseases and their associated viruses is usually performed by visual observation symptoms or using laboratory tests. can be carried out directly in the field, case manifest pathologies, means biological indexing with susceptible indicators, latent diseases. assays include various serological biomolecular tests, based on detection capsid nucleic acid, respectively. Serological are largely used, also thanks to availability commercial kits. Biomolecular underwent a dramatic evolution last years, from dot-blot hybridisation PCR-based techniques finally microarrays, improving sensitivity reliability.
springer.com
sci-hub.st 参考文章(55)
Varvara I. Maliogka, Chrysostomos I. Dovas, Nikolaos I. Katis, Generic and species-specific detection of viruses belonging to an evolutionary distinct lineage within the Ampelovirus genus Journal of Virological Methods. ,vol. 154, pp. 41- 47 ,(2008) , 10.1016/J.JVIROMET.2008.09.007
Donald J. MacKenzie, Morven A. McLean, Srima Mukerji, Margaret Green, Improved RNA Extraction from Woody Plants for the Detection of Viral Pathogens by Reverse Transcription-Polymerase Chain Reaction. Plant Disease. ,vol. 81, pp. 222- 226 ,(1997) , 10.1094/PDIS.1997.81.2.222
R KOENIG, H PAUL, Variants of ELISA in plant virus diagnosis. Journal of Virological Methods. ,vol. 5, pp. 113- 125 ,(1982) , 10.1016/0166-0934(82)90088-X
Michele Digiaro, Toufic Elbeaino, Giovanni Paolo Martelli, Development of degenerate and species-specific primers for the differential and simultaneous RT-PCR detection of grapevine-infecting nepoviruses of subgroups A, B and C. Journal of Virological Methods. ,vol. 141, pp. 34- 40 ,(2007) , 10.1016/J.JVIROMET.2006.11.033
Petr Komínek, Marcela Komínková-Bryxiová, Olga Mercedes Jandurová, Věra Holleinová, An RNA Probe for the Detection of Grapevine virus A Journal of Phytopathology. ,vol. 156, pp. 762- 764 ,(2008) , 10.1111/J.1439-0434.2008.01446.X
Annette Nassuth, Elisa Pollari, Katherine Helmeczy, Sandra Stewart, Sandra A Kofalvi, Improved RNA extraction and one-tube RT-PCR assay for simultaneous detection of control plant RNA plus several viruses in plant extracts Journal of Virological Methods. ,vol. 90, pp. 37- 49 ,(2000) , 10.1016/S0166-0934(00)00211-1
D. Zimmermann, G. Sommermeyer, B. Walter, M. H. V. Van Regenmortel, Production and characterization of monoclonal antibodies specific to closterovirus-like particles associated with grapevine leafroll disease Journal of Phytopathology. ,vol. 130, pp. 277- 288 ,(1990) , 10.1111/J.1439-0434.1990.TB01178.X
N Boonham, K Walsh, P Smith, K Madagan, I Graham, I Barker, Detection of potato viruses using microarray technology: towards a generic method for plant viral disease diagnosis Journal of Virological Methods. ,vol. 108, pp. 181- 187 ,(2003) , 10.1016/S0166-0934(02)00284-7
L. TORRANCE, R. A. C. JONES, Recent developments in serological methods suited for use in routine testing for plant viruses Plant Pathology. ,vol. 30, pp. 1- 24 ,(1981) , 10.1111/J.1365-3059.1981.TB01218.X
N Habili, CF Fazeli, A Ewart, R Hamilton, R Cirami, P Saldarelli, A Minafra, MA Rezaian, Natural Spread and Molecular Analysis of Grapevine Leafroll-Associated Virus 3 in Australia Phytopathology. ,vol. 85, pp. 1418- 1422 ,(1995) , 10.1094/PHYTO-85-1418