The level of CRABP-I expression influences the amounts and types of all-trans-retinoic acid metabolites in F9 teratocarcinoma stem cells.

摘要: The CRABP-I and CRABP-II proteins are high affinity cytoplasmic retinoic acid-binding proteins. In undifferentiated F9 teratocarcinoma stem cells, only the protein is expressed at detectable levels. We have previously shown that overexpression of in stably transfected cell lines results a lower sensitivity to given external concentration acid relative untransfected cells; contrast, reduced expression cDNA anti-sense associated with increased these acid. These three types were cultured presence 50 nM [3H]retinoic acid, metabolism was followed over next 24 h. demonstrate has ability alter both levels RA metabolites produced cytoplasm differentiating embryonic cells. Moreover, level determines rate 4-oxo-RA such higher level, faster This first reported connection between intracellular metabolism.