Lymphocyte apoptosis during the silencing of the immune response to acute viral infections in normal, lpr, and Bcl-2-transgenic mice.

摘要: This report examines the mechanisms involved in down-regulation of immune response acute viral infection and documents presence apoptotic lymphocytes situ spleens mice during resolution to lymphocytic choriomeningitis virus infection. Apoptotic cells were detected by an nucleotidyl transferase assay. Both T B shown be dying vivo, latter clusters. A biphasic occurrence apoptosis course was observed, with elevated levels occurring at day 3 after a second more pronounced peak 11 infection, coincident decline cytotoxic lymphocyte decrease total splenic leukocyte number. Apoptosis vivo lpr mice, suggesting that Fas expression is not imperative for context unaffected enforced lymphocyte-directed Bcl-2, protein blocks growth factor deprivation-induced vitro. These results argue silencing cell may result simply deprivation. The susceptibility activated death, which has previously been associated virus-induced deficiency, therefore also explain en masse elimination expanded pool subsequent