Circulating DNA in systemic lupus erythematosus

摘要: Abstract Circulating double-stranded DNA (dsDNA) in the form of immune complexes has long been thought to play an important pathogenetic role systemic lupus erythematosus (SLE) glomerulonephritis. However, attempts demonstrate circulating patients with SLE have led conflicting conclusions, largely for technical reasons. Using methods designed avoid these limitations it was previously shown that occurs infrequently if at all normal subjects. Surprisingly, same reported be true unselected although sensitivity assay used relatively low. Preliminary experiments this laboratory, using improved sensitivity, suggested association between dsDNA and uncommon manifestations vasculitis and/or central nervous system (CNS) involvement. In present study possibility on explored by prospectively classifying active into those vasculitis, CNS involvement or neither. Patients other clinical states which themselves are known believed associated were excluded. Multiple plasma specimens from each patient examined a modified counterimmunoelectrophoresis (CIE) capable detecting 20 50 ng/ml dsDNA. considered only when latter demonstrated multiple separate occasions. Of 16 had persistently DNA. contrast, one 18 but without DNA, highly significant difference. This also seen longitudinal studies four individual who episodes activity, some included vasculitis. not found inflammation alone receiving corticosteroids cytostatic agents. Similarly, differences prevalence anti-dsDNA antibodies groups could account results. Hence, is concluded specifically Furthermore, lack appears negligible within limits used. Possible implications results discussed.