Appearance of circulating DNA during hemodialysis

摘要: Abstract The appearance of circulating DNA during hemodialysis was investigated by use a modified counterimmunoelectrophoresis assay capable detecting as little 0.02 to 0.05 μg native per milliliter plasma. It found that free regularly released the procedure in widely varying amounts which ranged high 5 μg/ml. Both amount plasma and its rate increased progressively throughout had no temporal correlation with transitory early leukopenia previously described. Circulation vitro fresh blood through dialysis coil also resulted suggesting passage itself can account, at least part, for DNA, presumably release from leukocytes. Neither presence (nor reversal) uremic state se nor removal inhibitors are able account findings. Whether is clinical importance is, yet, unknown. A hypothesis proposed linking chronic exposure intracellular materials, including accelerated atherosclerosis recently described late complication hemodialysis. Currently, only known be characterized over long periods time. Since this phenomenon iatrogenically induced, further exploration possible pathogenetic implications seems particularly important.