Immortal brown adipocytes from p53-knockout mice: differentiation and expression of uncoupling proteins.

摘要: Abstract Brown adipose tissue (BAT) is the specific site for metabolic heat production in mammals. To establish a novel immortal brown adipocyte cell line, stromal-vascular fraction containing preadipocytes was obtained from interscapular BAT of mice deficient tumor-suppressor gene p53. The p53 -deficient cells, tentatively named as HB2 could be cultured vitro after repeated passages and differentiated into adipocytes presence insulin, T 3 and/or troglitazone, expressing some adipocyte-specific genes accumulating intracellular lipid droplets. mRNA level uncoupling protein 1 (UCP1), mitochondrial specifically present adipocytes, undetectable preadipocytes, but increased differentiation. In UCP1 expression markedly activated stimulation β-adrenergic receptor pathway. UCP2 UCP3, recently cloned isoforms UCP1, were also detected their levels not influenced by adrenergic stimulation. Thus cells seem useful studies UCP functions.